Glutathione S-transferase and cytochrome P450 monoxygenase activities of Bradybaena similaris treated with metaldehyde

S. Salmijah, S.Z. Syed Zahiruddin and B.S. Ismail, School of Environmental and Natural Resource Sciences, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43600 UKM, Bangi, Selangor, Malaysia.


The snail Bradybaena similaris was given metaldehyde bait at 0 (control), 1% and 5% for 24 hours. Glutathione S-transferase (GST) activity, total cytochrome P-450 hemoprotein content, and two cytochrome P-450 isoenzymes, CYP1A1 and CYP2B1 were assayed at 0, 24, 48, and 72 hours after treatment. The GST and CYP2B1 activities in the treated animals increased significantly (P <0.05) compared to the control animals. CYP1A1 activities were however not affected. These observations suggest that a single exposure to metaldehyde can cause increases in the xenobiotic metabolizing enzymes, particularly GST and certain cytochrome P-450 families, which can eventually lead to resistance in the animals.


Plant Protection Quarterly (2004) 19 (4) 151-154.